Sample Ap Free Response Essay Prompts Are Highly Effective For Improving Your Writing Skills

Sample Ap Free Response Essay Prompts Are Highly Effective For Improving Your Writing SkillsTo create effective responses to sample a free-response essay prompts, you will need to know what they are and how they work. In this article, I will help you understand what sample a free-response essay prompts are, and what you need to do in order to help improve your writing skills.It is a common myth that it is impossible to write good essays on a blank slate. While that is not completely true, this particular myth is certainly false. The point is that if you have no written experience to fall back on or have some sort of experience that is less than satisfactory, it can be extremely difficult to write well when faced with a blank sheet of paper.The way in which you respond to sample a free-response essay prompts is much the same as the way in which you would respond to any other type of essay prompt. You simply write a set of sentences, or paragraphs, that illustrate your argument. The po int is to use all of the skills you have to be able to demonstrate your case effectively in a manner that is both logical and compelling.There is nothing wrong with a person being taught by a teacher, or being given the benefit of hindsight, but this is not the case when you are faced with a blank slate. This means that you must be willing to go beyond just following what you have been told in your school's lesson plans or being taught by the same teacher who gave you those lessons.The truth is that writing in a unique and original way is always easier on a blank slate. Your writing must be able to stand on its own without any doubt or self-consciousness. If you look at some of the better essay prompts you will see a certain style that stands out above the rest of the bunch.The best response essay prompts will be able to let you put yourself in the role of the student. You must be able to show that you are in their shoes, and you should use every ounce of your energy to show that yo u have learned something new from the lesson that you have taken. The key is to be honest about the lessons that you took, and to do that effectively, you will need to be honest about the way you write.For a brief time, the use of sample ap free response essay prompts may have had a tendency to turn people off from writing for a living. However, this is largely an era gone by, and the fact is that there are people who want to write essays for a living. The idea that you must become a master in order to write for a living is a myth, and it is more important that you learn to be honest with your writing.

My Opinion on the Movie Titanic

The essay is an analysis of the movie Titanic written and directed by James Cameron and was released on 19 December 1997 and runs for 194 minutes. The movie is a flashback an epic romance and tragedy, telling a story of Lovett who hunts for treasure. Together with his squad, he looks for heart of the ocean; a necklace they deemed was in Cal’s safe. What they found was a portrait of a woman dated 1912 14 April.Advertising We will write a custom essay sample on My Opinion on the Movie Titanic specifically for you for only $16.05 $11/page Learn More Upon the knowledge of the portray, Dawson Calvert contacted Lovett, she was asked if she knew about the necklace and she said that indeed she was the one Rose DeWittone of the passengers deemed to have die when the ship sunk after hitting a iceberg. She retells how she boarded the ship in a first class with her fiancà © Cal a son of Pittsburgh rich man. The latter’s parents stressed the importance of the two to marry. Being disturbed, Rose contemplated committing suicide. Just before she slips off the ship, Jack comes into the scene and convinces her not to do that. The two later established developed a mutual relationship. Cal together with his mother did not approve of this kind of friendship but Rose defied this kind of orders (‘Titanic’). One moment she and jack found their way out of sight of Cal’s bodyguard, they went to room where Rose told Jack to draw her nude but putting on the necklace. It is later that thy made love. Cal discovered the portrait drawn by Jack. Cal framed Jack by lacing the necklace in Jacks coat who was later arrested. He was handcuffed. At this moment the ship had hit an iceberg and the ship was sinking, Cal, his mother and Rose were on board, the later escape to go and try to save Jack. The two later managed to get back to the deck, at this point, Cal made Rose to board the lifeboat again with a promise that both Jack and Cal will be safe, the later then double-crossed Jack. Seeing this Rose found her way out to be with Jack in the sinking ship. With frustration, Cal ran after them while shooting. Shortage of ammunitions made him return and boarded the lifeboat. While Jack and Rose were at the top of the deck people were dying since all life boats had disappeared. A panel that could support one person is what helped them, Jack died as a result of hypothermia. With the return of a lifeboat, Rose signaled the crew using a whistle; she is saved and goes to New York where she changed her name to Dawson Rose. She later went to Lovett’s ship and dropped the necklace. This is one movie I like due to the romance, music soundtrack, the issue of social class. The kind of music played in the movie left me more connected with it, the song from Celine Dion â€Å"my Heart Will Go On† is in line with the love theme. Additionally, when the ship had hit an iceberg, the bandleader Henry called up his eight man team and started playing waltzes and ragtime.Advertising Looking for essay on art and design? Let's see if we can help you! Get your first paper with 15% OFF Learn More They all perished while sinking this was very touchy (‘Titanic’). Another music that made me glued and really felt what was happening in the movie is a track titled, â€Å"Death of Titanic† this was an anticipation of what would happen later in the ship. The loud cries of those falling for death after the departure of all the lifeboats are the climax. The romantic scenes in the movie were a blast in my opinion. Romance started when Jack arrived just in time to persuade Rose not to commit suicide by falling from the ship. After that scene, it is evident that the two crafted there way out of Cal’s bodyguard. In a room, Rose demanded to be drawn while putting on nothing other than there necklace. Similarly, there are other brief scenarios where the two made love, in the deck. It is indeed this love that makes each open yearn for the safety of the other. On the same note, the incident where jack and rose dance was also full of romantic expression. This made me and I bet more individuals who hold the same opinion like that of mine to love this movie. The death of Jack while with Rose is another memorable moment in this movie. Lastly, the issue of social stratification did come out clearly as it was well planned by Cameron. In any given set of a society, it is apparent that all individuals who constitute that society cannot be in the same class. It is for this reasons that most of those aboard the ship lost their life. Majority was third and second class who could not be allowed to enter the lifeboats, those individuals in first class were saved (‘Titanic’). Additionally, Jack’s relationship with Rose was not approved just because he was from a lower class than that of Cal whose marriage with Rose was purported to solve the financial pr oblem her family had. From the review of what makes me love the movie Titanic, are three main things the romantic scenes, the issue of social class and the sweet sound tracks and music. Works Cited ‘Titanic’. Dir. Cameron, James. Prod. Sanchini, R. Videocassette. Paramount Pictures, 1997Advertising We will write a custom essay sample on My Opinion on the Movie Titanic specifically for you for only $16.05 $11/page Learn More This essay on My Opinion on the Movie Titanic was written and submitted by user Samantha Burt to help you with your own studies. You are free to use it for research and reference purposes in order to write your own paper; however, you must cite it accordingly. You can donate your paper here.

cat vs dog essays

cat vs dog essays There comes a time in most everyones life when he or she decides to buy a pet. Some people go for the unusual and choose a bird, snake, or rodent. Most people, however, decide on the more common four-legged creatures like a dog or cat. Dogs and cats are very different animals and they have different attitudes, needs, and habits. Understanding these differences can help in the process of choosing between them. For unwavering love and loyalty, not to mention protection, a dog is the choice. Dogs will not question authority, and they will, after some training, do exactly what they are told to do. There are many species of dogs, and they come in large, medium, and small sizes. Smaller sizes like poodles and bichons for people who just need an extra friends and bigger dogs like rottweilers and mastiffs for those who are in need of further protection. Dogs require a lot of attention, and they will make sure their owners are aware of it. They need to be watched constantly and must be taken regularly for walks, because, as most owners know, a dog cannot be trained to do his business in a litter box. A dog will, however, respond instantly to his owner's every wish and will lie at or on that owner's feet anytime, anywhere. A dog is very much like a child in that it cannot be left alone in the house for too long. It will get bored easily and can, without too much effort, make your house look as if a tornado were just there. On the other hand, for ease of care, or peace and quiet, cats can be a better choice. Cats will not question authority; they wont even listen to it. There are many species of cats, but their size will generally be the same for all breeds. They will only sleep on the bed if they want to, and will sleep at whichever end they choose. Cats are quite independent, and they do not require much attention over and above regular feeding and regular cleaning of the litter box. It is a c ...

Managerial report on design of Honda Element Essay

Managerial report on design of Honda Element - Essay Example The rear doors open in a suicide way even though the suicide doors open independent from the front doors. To be on the safe side the front doors should be opened first to open the rear doors. After the doors are opened the interior is totally spacious. There is plenty of rear sear room and these seats can be folded either up, to the side, even folded down or the most astonishing part removed totally. The Element being motivated by Box on wheels the headroom is very plentiful. The front seats are very comfortable and there is ample amount of space given. The hatch opens in two sections in which one door flips down and the other flips up. This is also a utility and loading stuff is easy. With the hatch just being opened the lifting of stuff is very low. The theme of the car's interior is somewhat rugged. The floor is not covered in carpet and the entire floor is covered with a material, which does not absorb any moisture. Dirt, sand and even mud wipes totally. To achieve a rugged interior Element unlike other SUV's does not come with a hard plastic. A rubber like material covers the door panels and the dashboard area and it feels as if this material will come up against all kinds of punishment without scratching, cracking, denting or fading. The Element is an unusual vehicle with its utilitarian mode for Honda but they have come up with a good SUV. Element has a functional interior and combining that with its exterior design it has the features of a SUV and a pick up truck and is a target for the young and active buyers. It has been designed to serve also as a base camp, room for the young buyers. The Element can hold up to four adults and they can have a splendid journey. With the rear seats easily folded anything can be put in for the weekend trip. The interior has been designed in such a way that it could be easily cleaned. There are some additional features too including an AM/FM radio with a 6 CD Changer and a MP3 player, which has video game outlets. Honda has never excelled in its audio system area. The previous Honda systems were just a simple four-speaker system, which sounded tiny and flat. With Honda knowing the target market for Element, there was a big no for a simple and tiny stereo. The end result was in the Element EX, Honda put in a mid range, nice system with a good bass, treble and sub woofer. The system, which is a 270-watt, uses 7 speakers and gives out a deep bass, clean and a brilliant sound. Additional bonus is the adjustable sub woofer. When driving the Honda Element it feels as if driving a Jeep of Sorts. The windscreen, which is upright and seat position, which is very high, it gives a good view of the road with out sacrificing the interior understanding. The Gauges are arranged in three circles, which are similar to a Pontiac Vibe, but Honda has finished the rings in a silver matte rather than shiny chrome found on the Pontiac. Every thing in the Element has quality, feel and a nice variety of textures, which work together to drive the concept theme of a car. The Element is powered a 2.4 liter, inline 4, I-VTEC engine which produces 160 horsepower. The Element is not a sports car but there is sufficient power available for many driving situations. The Element never feels underpowered. The 5-speed manual

Ontario and Forest Sustainability Essay Example | Topics and Well Written Essays - 2750 words

Ontario and Forest Sustainability - Essay Example Although Canada is often deemed, from a romantic point of view, as a source of endless beauty and natural resources, this is simply not the case. There are limits. The Ontario forests are owned primarily by the province of Ontario, under the auspices of the "Crown Forest", and represent more than ninety percent of all forested land in the province; of these forested lands, slightly more than thirty percent have been allocated for productive uses. These productive uses include the cutting down of timber, tourism, and a variety of other uses. Because these forested lands provide different benefits to different groups, both aesthetic and economic, philosophical conflicts are inevitable. The provincial Ministry of Natural Resources of Ontario asserts that it is doing everything possible to sustain the forested lands; and, yet, there are those that counter that more needs to be done given certain data suggesting that sustainability problems have occurred and continue to emerge. The questi on presented, in short, is whether Ontario is doing enough, both in terms of policy formulation and implementation, in order to truly ensure sustainability. I am of the view that, while Ontario's actions seem superficially comprehensive and cohesive, the fact is that there remain very real problems regarding the forests and related industry. How might Ontario better approach this issue of sustainability I will attempt to provide some answers by discussing the goals and types of sustainability, some of the policies and programs adopted in Ontario, and the ultimate reality on the ground. Sustainable Development Defined As a preliminary matter, it is important to define what is meant by the term, sustainable development. The most widely accepted definition has been attributed to the Bruntland Commission which stated that sustainable development is that type of development that "meets the needs of the present without compromising the ability of future generations to meet their own needs."1 This implies a policy model that emphasizes regeneration; more specifically, sustainable development demands that our actions today do not disadvantage people in the future. The difficulty is that sustainability must account for several sub-types of sustainability; these sub-types include environmental sustainability, political sustainability, social sustainability, and economic sustainability. One can easily imagine the conflicts which arise when one group tends to emphasize environmental sustainability, for instance, and another group chooses instead to emphasize political sustainability given certain economic p ressures and rewards. These competing points of view have been stated as philosophies or ideologies governing how policy should be inspired and designed. The ecocentric school of thought treats everything as an interrelated living whole and posits that all policy decisions must account for all types of sustainability2; the anthropocentric approach, on the other hand, holds that the well-being of human beings must transcend all other concerns.3 Many critics have argued that the anthropocentric approach has resulted in legislation and policies which have exacerbated rather than minimized certain sustainability concerns. An

An aseptic technique

An aseptic technique Introduction Aseptic means to be free from microorganisms. Aseptic Technique is the procedure that is performed under sterile condition to prevent the growth of other microorganisms on the growth medium such as the Petri dishes containing the nutrient agar or the pure culture. If the growth medium or the pure culture is contaminated with microorganisms from the environment, it will results in confusion and inaccurate data. Hence, it is important to reduce the risks of these microorganisms to come in contact with the experimental materials. In addition, by maintaining a clean environment when transferring the culture of microorganism onto the nutrient agar is part of the aseptic technique. This is usually done by disinfecting the table before and after working with microorganism using alcohol. Flaming the experimental materials such as bacteriological loops, bottle or flask necks can help in sterilizing. It must be done for several seconds so as to raise the temperature to kill the contaminants; however, the bacteriological loop must be cooled for a while before it is used to pick up the microorganism as picking up microorganism with a hot tool will kill the cells. When removing caps from bottles, it is important to keep the cap in the hand as by putting them on the table, it will be contaminated. Flaming is also required before replacing the cap onto the bottle. It is important to handle open tubes at an angle so that airborne and other microorganisms will not fall into the tube and cause contamination. During streakin g, it is important to keep the lids of the Petri dish over it to prevent contamination. Lastly, try to avoid breathing, coughing, sneezing and talking while transferring the culture so as to reduce the risk on contaminating. Apart from this general aseptic technique, there also several other methods to ensure that destruction of living microorganisms in materials and apparatus. One of the methods is by using dry heat which is sterilising using naked flame or hot air. Sterilising materials or apparatus by a naked flame is usually heated to redness and allowed to cool. They are usually made of metal. Exposure to hot air helps to destroy microorganisms in glass and porcelain apparatus. The other method is sterilizing using moist heat which can be used in three different ways which are heating in water or steam at 100oC, heating in steam under pressure and discontinue heating at low temperature. The different ways are employed according to the different materials or apparatus used. The last method is by using chemical, it can be either in liquid or gaseous state. They are often used in the disposal of contaminated materials and apparatus after a laboratory session. Microorganisms worked with in a lab should not be released into the environment as these strains may contain genetic markers such as antibiotic resistance. Therefore, they must be discarded properly. In addition, aseptic technique is not only applied in laboratory, it is also applied in clinical and surgical setting. Aims There are two aims in this experiment. The first aim is to show that a large number of microorganisms exist on the surface of our hands. The second aim is carry out the aseptic technique properly by transferring pure culture and inoculating them onto an agar plate. Materials and Methods Bacteria on skin Please refer to the Laboratory Manual unless otherwise stated of changes made. Streak Plate Please refer to the Laboratory Manual unless otherwise stated of changes made. A disposable sterile bacteriological loop is use instead of the metal sterile bacteriological loop so no heating is required. Discussion Bacteria on skin The human skins surface do carry a large number of microorganism and that by washing hands, individual can reduce the number of microorganism noticeably. However, even after a hand wash, microorganisms are still present on the surface. Streak Plate By employing the streaking technique on an agar plate correctly, a single colony can be obtained. Furthermore, it can be used to separate colonies of mixed culture. Hence, this pure colony can be picked up and to be grown in large quantity. From the result above, it can be observed that single colonies of the S.aureus are found. Due to the colour and morphology, it can be noted that the S.aureus is of a pure culture. Conclusion Aseptic technique is a basic laboratory technique that must be employed especially during Microbiology laboratory session so as to prevent any contamination and affecting the accuracy of the result. Since microorganism can replicated rapidly, disposal of contaminants must be done properly so as to protect both the equipments and the health of individuals. B- Gram Staining Introduction Gram stain is also known as differential stain in which it will divide bacteria into two large groups, mainly Gram Positive and Gram Negative. This difference is due to the chemical and physical structure of the cell wall called peptidoglycan. During solvent treatment, if the peptidoglycan is able to retain the crystal violet dye, the bacteria will be group as Gram Positive bacteria. However, if it is not able to retain the crystal violet, the bacteria will be group as Gram Negative bacteria and that it will be stained pink. Gram Positive bacteria has a thicker peptidoglycan (50-90% cell wall) as compared to the Gram Negative bacteria (10% cell wall). In addition, the Gram Negative bacteria has another layer which is make up of liposaccharides and proteins and is separated from the cell wall by the periplasm. In gram staining, there are four basic steps which include flooding the heat fixed smear with crystal violet stain, following by the addition of iodine solution to form complex, adding of alcohol for decolourisation and counterstaining with safranin. After flooding the peptidoglycan with crystal violet stain, the dye will enter the cells and all cells will turn purple. With the addition of iodine, a crystal violet-iodine complex will be form such that it will not be able to exit the cells easily. By decolourizing the cell with alcohol, the peptidoglycan of the Gram Negative bacteria will break down because the alcohol will dissolves the liposaccharides layer and hence, with the removal of the layer, the crystal violet-iodine complex will run off which will results in the loss of the crystal violet stain and the cells turn colourless. On the other hand, the alcohol will dehydrate the Gram Positive bacterias peptidoglycan, closing the pores as the peptidoglycan shrinks. As a result, the crystal violet-iodine complex will not be able to run off as the exits will be blocked and they remained stained. By counterstaining with safranin, the Gram Negative cell will turn pink and the Gram Positive cells will remain violet. With gram staining, one is able to differentiate if the culture is a pure or a mixed, the morphological details of the bacteria and the arrangement of the bacteria. Aims The aim is to prepare smears for staining, observe the morphological details of the bacteria and to be able to differentiate between Gram Positive and Gram Negative bacteria. Materials and Methods Preparation of Smears for staining Please refer to the Laboratory Manual unless otherwise stated of changes made. A disposable sterile bacteriological loop is use instead of the metal sterile bacteriological loop so no heating is required. Gram Staining Method Please refer to the Laboratory Manual unless otherwise stated of changes made. Discussion According to the result observed, Bacillus subtilis is rod shaped (bacillus). They are stained purple which suggests that they are Gram Positive bacteria. They are arranged in singles. Although, endospore cannot be observe in this experiment, they can also be found on Bacillus subtilis. The endospore enables the bacteria to tolerate harsh environmental condition such as high temperature. Bacillus subtilis can also be known as a single bacillus bacterium. Escherichia coli is stained pink and thereby, it is a Gram Negative bacterium. The cells are also rod shaped but they do not have any particular cell arrangement. They are found in singles, pairs and even clusters. Proteus vulgaris is also stained pink and hence, a Gram Negative bacterium. Its morphology rod shaped and is arranged in singles. They can also be known as a single bacillus bacterium. Staphylococcus aureus is a Gram Positive bacterium as it is stained dark purple after gram staining. It has a spherical shaped, otherwise known cocci and they are usually arranged in grape-like clusters. Therefore, they are known as a staphylococci bacterium. There were no differences in the shape and colour observed for each of the bacteria, hence, they can be known as a pure culture. Conclusion The Gram staining method is a useful tool used in most laboratories as it helps individual to visualise the bacteria accurately and effectively such as the shape, arrangement and even whether the culture is a pure or mixed. However, it should be noted that not all bacteria will give a gram reaction as some of them are gram variable, otherwise known as gram indeterminate. Therefore, they will give a mix of pink and purple cells after gram staining. For some of the Gram Positive bacteria, their peptidoglycan breaks easily during cell division, hence, after staining, they will give pink cells instead of purple. In addition, the duration of a culture can also affect the gram stain. C- Cell Counting Introduction Cells counting is the accurate and precise counting of cells. They are usually carried out manually or electronically. By counting cells manually, a counting chamber, otherwise known as the haemocytometer is used. The counting chamber is used to determine the number of cells per unit volume of a suspension. On the other hand, a coulter counter is used to count cells electronically. There are two approaches to count the number of cells, mainly total cell counts and the viable counts. Total cell counts are counted directly using the microscope and that both living and dead cells are counted. This is normally accompanied by the use of the counting chamber or coulter counter. Another approach is the viable counts which only count the living cells. The small volume of culture, otherwise known as the dilution of the culture is applied to the surface of an agar plate. After incubating, the colonies are counted, normally colonies between 30-300 are chosen to be used for the calculation of concentration of the given sample. The units given is colony forming units (CFU) per ml. The haemocytometer is a modified glass slides with two count chamber of known area. Each chamber grid is composed of nine squares which are known as subgrid, each square is 1mm2. Within each large square, there are further sub divisions that help in counting. When the coverslip is placed over the grooves of the slide, there will be a thickness of 0.1mm. Hence, the volume is 0.1mm3 or 1 x 10-4ml. Therefore, the cell concentration will be calculated as the number of cells multiply by 1 x 104ml and again, multiplying the dilution factor. Since cells are very small and they can be observed in a very high number, the suspensions should be diluted enough so that the cells are able to distribute uniformly in the counting chamber. Aims There are two aims in this experiment. Firstly, to be able to determine the cell count in different biological species and secondly, to be able to determine the viable count of a live bacteria, Staphylococcus aureus. Materials and Methods Cell Counting using Counting Chamber Please refer to the Laboratory Manual unless otherwise stated of changes made. Serial Dilution is carried out before the sample is loaded into the Neubauer Manual Counting Chamber. Normal saline (0.9% NaCl) is used to dilute the blood and broth medium is used to dilute the brewers yeast ( Saccharomyces cervisiae). Both blood and brewers yeast are dilute in the ratio of 1:10 and 1:100. The 1:10 dilution is prepared by diluting the 10Â µL of whole blood or yeast with 90Â µL normal saline or broth medium respectively. The 1:100 dilution is prepared by extracting 10Â µL from the respective sample from 1:10 and adding 90Â µL of normal saline and yeast into the respective sample. Cell Counting of Live Bacteria, S. aureus (after Serial Dilution) Please refer to the Laboratory Manual unless otherwise stated of changes made. Two changes were made in Step 1 and Step 12 respectively. Only three nutrient agar plates , 10-3, 10-4 and 10-5 were labelled and culture in these dilutions were spread on the respective agar plates. A new spreader and pipette tip were used everything a different dilution culture was spread on the agar plate. Count the number of colonies on the three different agar plates. Choose the agar plate with colonies between 30-300 to calculate the concentration of the original sample. Discussion Using the counter chamber, individual is able to give a quick assessment on the number of cells given that all the procedure on preparing and loading the sample onto it. One of it is that suspension/sample is not mixed before loading. This is due to the fact that cells tend to settle at the bottom of the tube and hence, while pipetting the sample out from the tube, individual do not have the actual or accurate number of cells. Therefore, to get a uniform suspension for a more accurate result, mixing the tube before pipetting is recommended. In addition, it can also help in reducing the clumping of cells. Furthermore, improper filling of chambers can lead to inaccurate volume of suspension in the chamber and leading to inaccurate cell concentration. Improper filling of chamber includes overfilling or under filling of sample. Moreover, there must be a consistency in counting cells which is in contact with the boundary lines (ie. the three lines just outside the grid) or when the cells are clump together. Individual will have to determine which cells to count and which not to count especially a cell which is situated on a border such as if a cell has half of its area outside the border, individual do not count those cells. The other method of cell counting is the viable count where a single cell will give rise to a colony which is visible to the naked eyes on the agar plate. Therefore, by counting the number of colonies on the agar plate, individual is able calculate the cell concentration. However, only plates which have 30 to 300 colonies are used to calculate the cell concentration. In the result for viable count of S.aureus, the plate chosen was 10-5 because there was 82 colonies in one quadrant which is equivalent to 328 (82 x 4) colonies on the agar plate. Although, the number of colonies (328) exceed the number of colonies of 300 that we were supposed to chose, this 10-5 dilution plate has the closest number to 300. However, we should dilute even further because a single colony can have clumps or chain of cells in it and hence, resulting in inaccurate number of colonies/cells in which the actual number of cells should actually be more than the calculated number of cells. The advantage of viable cell counting is that the organism counted will be a positive one (ie. S.aureus) instead of any other organism as if there is contaminant, the morphology or colour will be different. Another disadvantage of viable cell counting, other than cells that clump together or have chains which will form a single colony, is that organism will only grow in condition which is suitable for their growth on the agar plate. Cell counting usually is accompanied by serial dilution as it is impossible to count the number of cells if the concentration is too high as it will lead to a very high number of cells. Conclusion There are several other methods, other than using counting chamber and viable cell count, to count cells in a suspension. However, they are the least expensive and is able to give accurate result in a very short period of time. References Abcam, 2009. Cell counts using a haemocytometer. Abcam plc. Source: http://www.abcam.com/index.html?pageconfig=resourcerid=11454 Accessed: 1 October 2009 George Xu, 2007. History of the Gram Stain and How it Works. University of Pennsylvania. Source: http://www.uphs.upenn.edu/bugdrug/antibiotic_manual/Gram1.htm Accessed: 1 October 2009 H. Kayser, A. Bienz, Johannes Eckert. M. Zinkernagel. 2005. Medical Microbiology. Thieme Stuttgart., New York. p. 264-270. Kenneth Todar, 2008. The Growth of Bacterial Population. Todars Online Textbook of Bacteriology. Source: http://textbookofbacteriology.net/growth_2.html Accessed: 6 October 2009 Linda B, Mary R, 2007. Aseptic Transfer. Austin Community College. Source: http://www.austincc.edu/microbugz/aseptic_technique.php Accessed: 3 October 2009 Steve Hogg, 2008. The Gram Stain. Newcastle University. Source: http://www.ncl.ac.uk/dental/oralbiol/oralenv/tutorials/gramstain.htm Accessed: 6 October 2009

All Quiet on the Western Front by Erich Remarque

All Quiet on the Western Front In the book â€Å"All Quiet on the Western Front† by Erich Remarque, the author uses nature, and comradeship, to describe what the characters are going through. Erich uses nature in several ways, such as describing how the soldiers are facing terrible hardships, also it reflects on their sadness, and provides a contrast to the unnatural world of war. The author also uses the theme Comradeship through all the horrifying pictures of death and inhumanity, he talks about when Paul and his friends pick on Himmelstoss and beat him.We think it’s funny because Himmelstoss deserves it for being rude to them, and Paul and his friends are just giving him what he deserves. As we start going farther into the book, we start to realize that beating on someone isn’t funny anymore. We read the how the soldiers feel after assaulting and killing other people, it gives us a disturbing thought about war. Erich shows the theme Nature in many parts of the book. In chapter 2, when Kemmerich dies Paul takes his identification tags and walks outside.He then says â€Å"I breathe as deep as I can, and feel the breeze in my face, warm and soft as never before. † (Remarque 33) This is one of many times, when nature has helped the men go through bad experiences, and help them move on. Nature also reflects the terrible sadness of the lost generation. In Chapter 4, Paul's company sustains heavy losses and a recruit is wounded so badly Paul and Kat consider killing him to end his suffering. The Lorries and medics arrive too quickly, and they are forced to rethink their decision.Paul watches the rain fall and says: â€Å"It falls on our heads and on the heads of the dead, up in the line, on the body of the little recruit with the wound that is so much too big for his hip; it falls on Kemmerich grave; it falls in our hearts. † (Remarque The cleansing rain falls upon the hopelessness of Paul's life and the lives of those around him. Throughout Remarque's book, we also see a strong affinity between nature and lost dreams and memories. When Paul is on sentry duty in Chapter 6, he remembers his childhood and thinks about the poplar avenue where such a long time ago they sat beneath the trees and put their feet in the stream.Back then the water was fragrant, the wind melodious; these memories of nature cause a powerful calmness and awaken a remembrance of what was but sadly, will never be again. Finally, butterflies play gracefully and settle on the teeth of a skull; birds fly through the air in a carefree pattern. This is nature in the midst of death and destruction. While men kill each other and wonder why, the butterflies, birds, and breeze flutter though the killing fields and carry on as if mankind were quite insignificant.Even at the end when Paul knows there is so little time until the armistice, he reflects on the beauty of life and hopes that he can stay alive until the laws of nature once again prevail a nd the actions of men bring peace. He describes the red poppies, meadows, beetles, grass, trees at twilight, and the stars. How can such beauty go on in the midst of such heartache? Remarque says that this novel â€Å"will try simply to tell of a generation of men who, even though they may have escaped shells, were destroyed by the war. If words can touch what men hold to be dear in their hearts and so cause them to change the world, this book with its words of a lost generation, lost values, and lost humanity is surely one that should be required reading for all generations. . When Paul and his friends waylay Himmelstoss and beat on him, we laugh because he deserves it and they are only giving him his due. As time goes by, however, the pictures of camaraderie relieve the terrible descriptions of front line assaults and death, and they provide a bright light in a place of such terrible darkness.A young recruit becomes gun-shy in his first battle when a rocket fires and explosions b egin. He creeps over to Paul and buries his head in Paul's chest and arms, and Paul kindly, gently, tells him that he will get used to it (Chapter 4). Perhaps the two most amazing scenes of humanity and caring can be found in the story of the goose roasting and the battle where his comrades' voices cause Paul to regain his nerve. In Chapter 5, Paul and Kat have captured a goose and are roasting it late at night.Paul says, â€Å"We don't talk much, but I believe we have a more complete communion with one another than even lovers have. We are two men, two minute sparks of life; outside is the night and the circle of death. † As he watches Kat roasting the goose and hears his voice, it brings Paul peace and reassurance. Over and over again, in scenes of battle and scenes of rest, we see the comradeship of this tiny group of men. Even though Paul counts their losses at various points, he always considers their close relationship and attempts to keep them together to help each oth er.In Chapter 9, when Paul is alone in the trench, he loses his nerve and his direction and is afraid he will die. Instead, he hears the voices of his friends: â€Å"I belong to them and they to me; we all share the same fear and the same life; we are nearer than lovers, in a simpler, a harder way; I could bury my face in them in these voices, these words that have saved me and will stand by me. † There is a grace here, in the face of all sorrow and hopelessness, a grace that occurs when men realize their humanity and their reliance on others.Through thick and thin, battle and rest, horror and hopelessness, these men hold each other up. Finally, Paul has only Kat and he loses even this friend and father-figure in Chapter 11. Kat's death is so overwhelming and so final that we do not hear Paul's reaction; we only see him break down in the face of it. There is such final irony in the medic's question about whether they are related. This man, this hero, this father, this life â €” has been closer to Paul than his own blood relatives and yet Paul must say, â€Å"No, we are not related. † It is the final stunning blow before Paul must go on alone.